Journal: Frontiers in Neuroscience

Article Title: Restoring morphology of light sheet microscopy data based on magnetic resonance histology

doi: 10.3389/fnins.2022.1011895

Figure Lengend Snippet: The failure of existing registration algorithms in the cerebellum and olfactory bulb. (A,B) DWI; (C,D) NeuN image after registration; (E,F) overlaid DWI/NeuN (specimen 191209). The left hand column shows the result of Elastix with rigid and b-spline registration and default settings. The registration errors in the olfactory bulb and brain stem are reduced but the errors in the dentate gyrus and cerebellum are significant (arrows in panel E ). The right hand column shows the result of ANTs with affine and SyN and default settings. There is a reasonable overlap in the dentate gyrus but significant mismatch in the cerebellum and olfactory bulb (arrows in panel F ).

Article Snippet: In later studies, we used DWI and NeuN because NeuN and Syto16 have similar contrast and the NeuN stain from LifeCanvas was more consistent. lists multiple stages starting with the global alignment progressing to local higher resolution details.

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Restoring morphology of light sheet microscopy data based on magnetic resonance histology

doi: 10.3389/fnins.2022.1011895

Figure Lengend Snippet: Light sheet microscopy of different stains and MRH of different contrasts. (A) Auto fluorescent, (B) NeuN, (C) MBP, (D) DWI, (E) FA, (F) RD scale bar is 2 mm (specimen 200316).

Article Snippet: In later studies, we used DWI and NeuN because NeuN and Syto16 have similar contrast and the NeuN stain from LifeCanvas was more consistent. lists multiple stages starting with the global alignment progressing to local higher resolution details.

Techniques: Microscopy

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

Article Title: Age-dependent Maintenance of Motor Control and Corticostriatal Innervation by Death Receptor 3

doi: 10.1523/JNEUROSCI.1928-09.2010

Figure Lengend Snippet: (A) Representative images of β-Gal staining in adult DR3ko and DR3het mice in different areas of the brain. Number of β-Gal staining cells increased in the cortex of DR3ko mice, particularly in prefrontal areas such as the anterior olfactory nuclei (Ai, ii) and generally across other areas such as the primary motor cortex (Biv, v), but remained high and unchanged in the dentate gyrus (Bvi, vii). Comparative expression is shown for the prefrontal (Aiii) and motor cortex (Bviii). Areas in which β-Gal expression was detected is shown for DR3ko in yellow and DR3he in blue. Filled areas show high density β-Gal staining. Areas from which high magnification sections are shown (Ai-ii, Biv-vii) are indicated by numbers with red squares for DR3het and green squares for DR3het animals. (C) DR3 expression in DR3het brain as detected using the β-Gal reporter. BG = Basal Ganglia; Co = Cortex; Cb = Cerebellum; DG = Dentate Gyrus; Hf = Hippocampal formation. (D) Expression of DR3 and TL1A mRNA in the indicated areas of the brain as measured by RT-PCR. GAPDH is shown as a loading control. (E) Microscopy showing neuron-specific DR3 promoter driven β-Gal expression in the dentate gyrus using immunofluoromicroscopy. Signals from β-Gal overlap with NeuN (Ei-iii), but not GFAP (iv-vi).

Article Snippet: Brain region volume and cell number was based on NeuN/TH staining and determined using the Olympus CASTgrid stereology system on a 1:12 series using previously described methods.

Techniques: Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Microscopy

Journal: Ultrasonics Sonochemistry

Article Title: A single low-energy shockwave pulse opens blood-cerebrospinal fluid barriers and facilitates gastrodin delivery to alleviate epilepsy

doi: 10.1016/j.ultsonch.2021.105730

Figure Lengend Snippet: Comparisons of histopathologic and immunohistochemistrical sections by blank, epilepsy, epilepsy with GTD only (Sham), and epilepsy with FSW-GTD (FSW-GTD) groups. (a) H&E stains; (b) TUNEL stains; (c) GFAP stains; (d) Caspase3-NeuN stains, arrowed is the Caspase-3-stained cell; (e) time responses of Caspase-3 density. *p, # p, ξ p < 0.05 vs. control, epilepsy + GTD, and epilepsy groups, respectively; ** p < 0.01 vs. control groups, respectively, in one-way ANOVA with the Tukey post hoc test. (n = 5).

Article Snippet: Peroxidase in situ apoptosis detection kits (TUNEL S7100), Glial fibrillary acidic protein (GFAP), hematoxylin and eosin (H&E), caspase-3, and NeuN stain kits were purchased from Merck Millipore (Darmstadt, Germany).

Techniques: TUNEL Assay, Staining